1. DNA-binding proteins such as H-NS and HU function in bacterial cells to A. ca
ID: 201181 • Letter: 1
Question
1. DNA-binding proteins such as H-NS and HU function in bacterial cells to
A. catalyze addition of nucleotides during DNA replication.
B. compact the chromosome and coordinate gene expression.
C. introduce supercoils into bacterial chromosomes.
D. scavenge nucleic acids from the environment.
E. "proofread" DNA during synthesis.
2. What type of enzyme in the replisome catalyzes addition of nucleotides to the growing DNA strands?
3. Regarding the enzyme in # 2,
How many of these would be required if a new round of DNA replication began before the first one terminated.
B. They are both double-stranded, but nonidentical, because of crossing over.
C. One consists of a double helix of two new DNA strands, whereas the other is entirely parental.
D. Each strand on each chromosome contains interspersed segments of new and parental DNA.
E. Each chromosome contains one parental and one newly synthesized DNA strand.
B. Small, uncharged molecules
C. Small, charged organic molecules (e.g., amino acids)
D. Anions (e.g., Cl– and HCO3–)
E. Monosaccharides (e.g., glucose)
B. attachment to and colonization of host tissues.
C. maintenance of ion gradients.
D. synthesis of ATP.
E. nutrient binding and transport.
F. DNA repair.G. motility.
Explanation / Answer
1) Compact the chromosome and coordinate gene expression - HU is a histone like protein which induces negative supercoiling into circular DNA in a bacteria that compacts the DNA. They resemble the histone H2B of eukaryotes. H-NS is histone like nucleoid structuring protein that regulates the gene expression in bacterial cells.
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