You conduct a transformation experiment using the pBR322 plasmid which has tetra

Question

You conduct a transformation experiment using the pBR322 plasmid which has tetracycline resistance. In this experiment, you added E. coli cells to a high salt solution and then put half into a tube with pBR322, and half into a tube without pBR322. After incubating them, heat shocking them, and then placing them on ice, you added X-gal to both tubes and incubated them again. You then spread cells from each tube onto a normal media plate or a plate that contains tetracycline. After incubating them overnight, what do you expect to see on the plate with tetracycline and without the plasmid?

A. A few colonies

B. No colonies at all

C. A complete lawn with many colonies

Explanation / Answer

B. No colonies at all

Bacteria without the plasmids cannot survive in the presence of tetracycline as they are susceptible to tetracycline. Only the bacteria with resistant plasmid can survive in the presence of tetracycline.

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