DNA libraries are useful for identifying and isolating eukaryotic DNA fragments
ID: 915207 • Letter: D
Question
DNA libraries are useful for identifying and isolating eukaryotic DNA fragments of interest in research. Select the ways in which genomic and cDNA libraries differ.
1. Genomic libraries contain DNA fragments from an organism's entire genome.
2. Genomic libraries are difficult to express in prokaryotic cells, which lack a splicing mechanism.
3. cDNA libraries contain more sequence information than genomic libraries.
4. cDNA libraries are the DNA fragment collections that are stored and propagated in host cells through cloning.
Explanation / Answer
1. Genomic libraries contain DNA fragments from an organism where as cDNA carries only expressed gene sequence.
2. Genomic libraries are difficult to express in prokaryotic cell, due to absence of splicing mechanism where as cDNA has only coding sequence therefore directly expressed in prokaryotic system
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