DNA Sequence GTACGGÇACCTTACGCTAGAGCTCCGCGCGTTACTGGTACGAT IATCCCGTGCAATGGGAACTCGA

Question

DNA Sequence GTACGGÇACCTTACGCTAGAGCTCCGCGCGTTACTGGTACGAT IATCCCGTGCAATGGGAACTCGAGGCGCGGATTCAGCATCCTAA i Single Strahd of DNA : Double-Stranded ONA i Oligonucleotides (Primers) Primers Bound to DNA strands 1. What is the sequence of Primer A? 2. What is the sequence of Primer B? 3. Which primer could be used together with Primer C to amplify a portion of the DNA sequence? Why would the other primers not work? How many base pairs long would the product (amplicon) be from a PCR amplification using Primers A and B 4. 5. What is the melting temperature of Primer E

Explanation / Answer

Answer

1. 5' TGGAATGCGATC 3'
2. 5' TAGCATGGTCATT 3'
3. PRIMER D -

Because other primers A and E amplify lower strand. Primer B amplify above strand in other region of DNA.
4. 36 basepair
5. TAAGTCGTAGGATT

Tm = 2 X (A+T) + 4 X (G+C)
   = 2 x 9 + 4 x 5
   = 18 + 20
   = 380C

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