DNA Sample #1 5’– T C C A G T G A T C T C G A A T T C G C T A G T A A C G T T C

Question

DNA Sample #1

5’– T C C A G T G A T C T C G A A T T C G C T A G T A A C G T T C G A T

3’– A G G T C A C T A G A G C T T A A G C G A T C A T T G C A A G C T A

DNA Sample #2

5’– T C A T C G A A T T C C T G G A A T C A G C A G A A T T C G C A T A

3’– A G T A G C T T A A G G A C C T T A G T C G T C T T A A G C G T A T

If both samples are treated with the restriction enzyme EcoRI [recognition sequence G’AATTC] then indicate the number of fragments and the size of each fragment from each sample of DNA. List the fragment sizes in order of largest to smallest.

Sample # 1                                                               Sample # 2

# of fragments:________                                      # of fragments:________

Sizes of fragments (largeà small):                     Sizes of fragments (largeà small):

Explanation / Answer

sample 1 2 fragments

smaller fragment larger fragment

5' TCCAGTGATCTCG 3' 5' AATTCGCTAGTAACGTTCGAT 3'

3' AGGTCACTAGAGCTTAA 5' 3' GCGATCATTGCAAGCTA 5'

sample 2 3 fragments

fragment1 fragment 2 fragment 3

5' TCATCG 3' 5' AATTCCTGGAATCAGCAG 3' 5' AATTCGCATA 3'

3' AGTAGCTTAA 3' 3' GGACCTTAGTCGTCTTAA 5' 3' GCGTAT 5'

fragment 2> fragment 1=fragment 3

fragment 1 and fragment 3 are of same sizes

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