DNA Replication Exercise B: DNA Replication (con\'t) 49 SSBP Helicase Completion
ID: 194431 • Letter: D
Question
DNA Replication Exercise B: DNA Replication (con't) 49 SSBP Helicase Completion stage of replication: DNA Polymerase I . Cytosine Adenine - Guanine DNA Polymerase I -Thymine .. DNA Polymerase II -Uracil .. DNA Polymerase lil -Deoxy ribose+primase Once DNA Polymerase ll pairs the majority of complementary D-nucleotides on the developing leading and lagging strand, the RNA primers are still present. Another DNA Polymerase enzyme, DNA Polymerase I reads both the leading and lagging strands from 5. to 3' of the parental strands replacing the R-nucleotides with D-nucleotides (Fig. 13). While the leading strand is synthesized continuously, there is still an RNA primer at the origin of the replication bubble. It is also replaced by DNA Polymerase ®-Ribose ··gae A. Telomerase . Phosphate HRIRHH11811H312153242HHR1124211 ll 3. What is the function of DNA Polymerase 1? Fig. 13. Completion phase of replication: DNA Polymerase L Completion stage of replication: Ligase After DNA Polymerase I replaces the RNA primers, there is no phosphodiester linkage between Okazaki fragments. Another enzyme, ligase, travels down the lagging strand creating a phosphodiester linkage connecting the Okazaki fragments (Fig. 14). 5' 2 In Fig. 14, simulate ligase by connecting the Okazaki fragments of the lagging strand. Fig. 14. Completion phase of replication: Ligase. 4. What bonds do ligase synthesize? www.thebiologyprimer.comExplanation / Answer
3) DNA Polymerase I Participates in the replication of prokaryotes. The functions of DNA Polymerase I are described as follows :
Catalyzes the Template-Directed Polymerization of Nucleotides into Duplex DNA in a 5´3´ Direction
It acts as a proofreading enzyme because of its exonuclease activity in 3'5 direction.
It helps in nick translation during DNA repair in 5'3' (forward) exonuclease activity.
4) Ligase are the enzymes that facilitate the joining of DNA strands together. It works by synthesizing phosphodiester bonds between nucleotides thus playing a role in repairing single-strand breaks in duplex DNA in living organisms.
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