A particular Eukaryotic gene has four exons and three introns. The 1st, 2nd, 3rd

Question

A particular Eukaryotic gene has four exons and three introns. The 1st, 2nd, 3rd and 4th exons are 538, 321 and 416 and 384 bp long respectively, while the 1st, 2nd and 3rd introns are 3.6 kbp, 1.9kbp and 2.2 kbp respectively.
a) It was discovered that this gene encodes not one, but three mature mRNA types, each producing a different polypeptide (Longest = polypeptide A, Second longest = Polypeptide B and the Smallest = Polypeptide C), due to alternative splicing. The largest mRNA is 1659 bp long and produces a 480 amino acid long polypeptide. The start codon is located 100 bases from the 5’end of the mRNA (consider that the start codon is AUG and it begins at the 101 base position).
Draw this mRNA and label the following:
I. Start codon
II. 5’ UTR (give the length of this region in # of bases)
III. The exact base position (give the base #, counting from the 5’end) of the
first base of the stop codon.
IV. 3’UTR (give the length of this region in # of bases)

Explanation / Answer

Final mature mRNA

            5'UTR                                                                                3'UTR (11to 30bp)

                                   Start codon                          Stop codon

5' cap-------------101bp- AUG---------------------------------1659bp-UAA-------------------------AAAAAAAAAAAAAA 3'

           5'UTR                                                                                              3'UTR (11to 30bp)

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