BIOLOGY LAB QUESTIONS=- HELP ME i converted the absorbance values into CELL CULT
ID: 35262 • Letter: B
Question
BIOLOGY LAB QUESTIONS=- HELP ME
i converted the absorbance values into CELL CULTURE by multiplying each absorbance values by 1E10.
I need help with this. PLEASE SOLVE PART B. thanks. its says N= (log Nt- log No)/ log 2
b. How many generations of cells have been produced in Cuhure X and Culture Y within 120 minutes (1.25 points)? NOTE: # of generations in Culture A: N = (log N1 - log N0)/log 2 N = Number of generations in a given amount of tune log N0 = number of ccl Is at a early time point in the exponential phase log N1 = number of cells at a later time point in the # of generations in Culture B: exponential phase t = time in minutes between N0 and N1Explanation / Answer
Based on the given data, culture X:
Time
Culture X
0
100000000
20
2.700000000
40
4.500000000
60
5.500000000
80
1000000000
120
2100000000
The doubling time is 27.3
Growth rate is 0.0253
Equation: amount=147940134.1348*e0.0224*time
At t=0, calculated cell concentration = 147940134.1348
For culture Y is:
Time
Culture Y
0
100000000
20
3.200000000
40
6.500000000
60
1200000000
80
24000000000
120
4900000000
Doubling Time: 21.37
Growth rate is 0.0324
Equation : amount=64638668771.589*e-0.0162*time
At t=0, calculated cell concentration = 64638668771.589
Time
Culture X
0
100000000
20
2.700000000
40
4.500000000
60
5.500000000
80
1000000000
120
2100000000
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