BIOL 371 Microbiology alas Figure 1. Mutated genes present in twelve amdinocilli

Question

BIOL 371 Microbiology alas Figure 1. Mutated genes present in twelve amdinocillin resistant clinical isolates. The far left column lists the mutated genes and the top row describes the strain name for each clinical isolate. Gray shaded boxes indicate the individual genes mutated for each clinical isolate. FIG 1 Putative Amd resistance-encoding genes in clinical strains. Genes marked by gray shading have a mutation that is not present n any of the reference genomes. Specific mutations are listed in Table Table 5. Amdinoc n susceptible and resistant clinical isolates, relevant phenotype, growth in various media, and MIC on Mueller-Hinton (MH) plates TABLE 5 Effects of cysteine addition on Amd resistance of clinical isolates Growth on MM. Amd MIC (mgiite Strain Relevant phenotype M90 Cys No Cys 0.3 mM Cys 0.75 mM Cys DA5438 Yes Yes 0,125 0.125 DA28439 No Yes 0.25 DA14704 No Yes 0.125 DA14709 No Yes 0.38 DA14710 No Yes 0.125 DA14712 No Yes 32 0.5 DA14713 No No 0.125 Yes DA14715 No 0.19 DA14718 No Yes 0.38 DA14719 No Yes 0.5 0.094 DA24678 No Yes 0.38 DA24686 No Yes 32 16 0.19 DA24690 No Yes 24 0.125 DA24692 No Yes 0.19 Shown are growth on M9 glucose minimal medium (MM) with or without cysteine added and Amd Etest MICs on M plates with different concentrations of cysteine. DA5438 is

Explanation / Answer

The amdinocillin resistant clinical isolates became susceptible to amdinocillin because the cys+ in wild type strains was imparting resistance to the drug amdinocillin and growth was exhibited. Thus we can conclude that since the mutated cysB gene that was present in all the strains led to loss of growth due to increased susceptibility to antibiotic amdinocillin, the cysB gene is responsible for amdinocillin resistance.

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