BIOL 3810 Molecular Cell Biology Laboratory will not 3. 50 mL of Buffer I (for p
ID: 277912 • Letter: B
Question
BIOL 3810 Molecular Cell Biology Laboratory will not 3. 50 mL of Buffer I (for protein isolation): 0.01 M KHaPOs bufler, pH 7.5, containing 0.3 mM EDTA, and 30 grams/liter of You will be proúded with a 0.1M solution of KHaPOs buffer and a 0.5M EDTA solution. Ater preparing your solution, give it to the instructor who will combine all the solutions. Note: you wil need to adjust the pH of this solution-refer to Information Sheet B 4 75 mL 10X 1BE (Tris base, Boric acid, EDTA) -0.9 M Tris, 0.889 M boric acid, 20 mM EDTA. Romember that a 0.5M EDTA solution is provided. Ater preparing your solution, give it to the instructor to collect with solutions from the other groups. 5. 500 mL of Buffer A (for protein isolation): 10mM Tris, 3mM EDTA, pH 8.0. Remember that a 0.5M EDTA solution is proided. After preparing your solution, give it to the instructor to collect with solutions from the other groups. Note: you wil need to adjust the pH of this solution!-refer to Information Sheet B.Explanation / Answer
1) C1V1 = C2 V2
0.1 * X = 0.01 * 50
X = 5 ml.
C1V1 = C2 V2
0.05 M* X = 0.3 mM * 50
X = 300 ul.
PVP ( 30 gm / L)
1.5 gm / 50 ml.
5 ml of 0.1M KH2PO4+ 300ul 0.05M EDTA+ 1.5 gm PVP. volume make upto 50 ml.
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