Molecular Biology BIO327 Final exam Part A: Multiple choices (2 pts each 1. A ty

Question

Molecular Biology BIO327 Final exam Part A: Multiple choices (2 pts each 1. A typical polycistronic mRNA MAY contain all of the following EXCEPT (A) a leader region (B) start codons (C) stop codons (D) a ribosome binding site (E) a S' cap Use the following choices to answer questions 2-5 (A) Synthesizes a string of AMP in mRNA (B) Synthesizes RNA using an DNA template (D) Synthesizes DNA using an DNA template (C) Synthesizes DNA using an RNA template (E) Synthesizes RNA using an RNA template 2. What is the function of PAP? 3. What is the function of reverse transcriptase? 4. What is the function of RNA polymerase? 5. What is the function of DNA polymerase? 6. Which of the following is NOT a possible component of a DNA molecule? (A) Adenine (B) deoxyribose (C) cytosine (D) phosphate (E) uracil 7. A eukaryotic structural gene has two introns and three exons: 5'-exon1-intronI-exon2-intron2-exon3-3' The GU at the S' end of intron2 has been mutated so it is no longer recognized. What would the mature mRNA look like in the mutant? (A) S-exon1-exon2-exon3-3' (B) 5'-exon1-intron 1-exon2-exon3-3 (C) 5'-cxon1-exon2-intron2-exon3-3 (D) 5-exon1-intron1-exon2-3' (E)5'-exon2-intron2-exon3-3

Explanation / Answer

1. The answer is E. a 5' Cap.

5' capping is a post transcriptional modification of HnRNA or pre mRNA, which is a characterstic feature of eukaryotes. As the translation occurs along with transcription and there is no intron sequences in the prokaryotes, there is no post transcriptional modificaiton in prokaryotic gene expression.

2. What exactly PAP is...as there are several abbreviations such as Poly A polymease etc... the function is depends on the enzyme. provide the full name in comments the i can help you.

3. The main function of reverse transcriptase is, it synthesizes the DNA from mRNA, this DNA is called complementary DNA. It plays crucial role in retro viral reproduction.

4. The main imporation function of RNA pol. is synthesis or RNA from DNA template, it is called Transcription and it is the part of gene expression.

5. The main important function of DNA pol. is sunthesis of new or daughter DNA strand. It is called replication.

6. The answer is E. Uracil. The Uracil is present in RNA and absent in DNA where as the thymine is presnt in DNA and absent RNA.

According to chegg guidelines we have to answer one question at a time.So, post the rest as seperate questions then I can help you.

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