Human cancer cells were treated with either EGF (+) or vehicle control (-) for 2
ID: 146377 • Letter: H
Question
Human cancer cells were treated with either EGF (+) or vehicle control (-) for 20 minutes. Below are the results of an SDS-PAGE followed by a specific immunoblot with an antibody specific for Phosphorylated-Tyrosine at residue 1068 of EGFR (upper) and another immunoblot with an antibody specific for EGFR (lower).
a.) Label each of the three columns of the blot.
b.) Interpret these results based upon protein size.
c.) Interpret the overall impact of EGF incubation on these cells.
tDa Phospho-EGFR (Tyr1068) 200 140 100 60 50 EGFR 140 100 80 60 50 40 EGFExplanation / Answer
In the given SDS-PAGE we can see that there are immunoblots for specific proteins with antibody.
(A) in the upper page there are a long signal that denote the phosphorylate tyrosine residues is epidermal growth factor but in lower cell one is tyrosine phosphorylation single and one is another but show the presence of the receptor of EGF.
(B) in the first page the size of Receptor is full but it may be fregmented in second page so it's size is reduced
(C) overall result show that it it treat the Cell with EGF then its nind ninh its receptor and tyrosine is active site but if there are NO EGF then there are no tyrosine detection.
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