DNA topoisomerases play important roles in DNA replication and in supercoiling.
ID: 142079 • Letter: D
Question
DNA topoisomerases play important roles in DNA replication and in supercoiling. These enzymes are also the targets for certain anticancer drugs. Eric Nelson and his colleagues studied m-AMSA, one of the anticancer compounds that acts on topisomerase enzymes. They found that m-AMSA stabilizes an intermediate produced in the course of the topoisomerase's action. The intermediate consisted of the topoisomerase bound to the broken ends of the DNA. Breaks in DNA that are produced by anticancer compounds, such as m-AMSA, inhibit the replication of the cellular DNA and thus stop cancer cells from proliferating Which of the mechanisms below describe how m-AMSA and other anticancer agents that target topoisomerase enzymes taking part in replication might lead to DNA breaks and chromosome rearrangements? Select all that apply. m-AMSA stabilizes the intermediate form of topoisomerase, which prevents the breaks from resealing, resulting in DNA damage m-AMSA binds to and stalls the movement of DNA polymerase, resulting in chromosome rearrangements m-AMSA stimulates the activated form of topoisomerase, which bubble, resulting in DNA breaks m-AMSA inactivates the proofreading ability of DNA polymerase, causes too many turns to be removed outside of the replication which can result in chromosome rearrangements m-AMSA inhibits the action of topoisomerase after the topoisomerase has generated double-strand breaks in the DNA. (E. M. Nelson, K. M. Tewey, and L. F. Liu. 1984. Proceedings of the National Academy of Sciences 81:1361-1365)Explanation / Answer
Answer:
The correct option is 1st and 5th
m-AMSA drug follow the following mechanism to stop the proliferation of cancer cells.
4'-(9-acridinylamino)- methanesulfon-m-anisidide (m-AMSA) works by blocking the enzyme topoisomerase II
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