From X-ray crystallography, you know two cysteins are 16.0 Angstroms apart. Doin

Question

From X-ray crystallography, you know two cysteins are 16.0 Angstroms apart. Doing FRET using two unknown fluorophores, you see a 38.5% energy transfer. What is the Forster distance of these fluorophores (in Angstroms)

* The answer I got for this was 14.7985 ( It was correct)

When you use known fluorophores, with a Forster distance of 18.0 Angstoms, you get a transfer efficieny of 90%. How far apart are the two cysteins, really?

*I got 12.4805 ( it was correct)

How do you reconcile this result with the X-ray crystallography- which is the BEST answer?

a)These values disagree- the protein has a different conformation in solution than in a crystal

b)These values disagree- the fluorophore is probably being quenched and this data is unreliable

c)These values agree within experimental error

d) They agree- this change could be relative fluorophore orientation

e)These values disagree- the protein is dynamic

I answered both A and B and they were incorrect.

Explanation / Answer

Answer will be A and D.

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