If the 10ml of solution factors in, how does this change the moles of Enzyme tot

Question

If the 10ml of solution factors in, how does this change the moles of Enzyme total? or how do you calculate the moles of Enzyme total?

Destroying the Trojan horse. Penicillin is hydrolyzed and thereby rendered inactive by penicllinase (also known as?-lactamase), an enzyme present in some resistant bacteria. The mass of this enzyme in Staphylococcus aureus is 29.6 kd. The amount of penicillin hydrolyzed in 1 minute in a 10-ml solution containing 10-9 g of purified penicillinase was measured as a function of the concentration of penicillin. Assume that the concentration of penicillin does not change appreciably during the assay [Penicilin] Amount hydrolyzed (uM) (nanomoles) 0.11 0.25 0.34 0.45 0.58 0.61 10 30 50

Explanation / Answer

* Calculate the number of moles of penicillin, which is 2.02 x 1012

* The turnover number is calculated as Kcat = Vmax/Et 0.68 X 10-9/2.02 X1012 = 3.37 X 102 mol/sec

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