4. (8 points) The ribonucleotides C and G were mixed together with C 2X more abu

Question

4. (8 points) The ribonucleotides C and G were mixed together with C 2X more abundant than G. The resulting RNAs were translated in a cell free system.

A. (5 points) Give the amino acids and the relative percentages of each that would be found after the translation. Show all reasoning/calculations.

B. (3 points) Suppose that di-ribonucleotides (GC) were used to make RNA and then translated in vitro. What would the results be – explain all reasoning and explain how this information could be combined with the information in part A above to try to deduce the genetic code, assuming you didn’t already know it.

Explanation / Answer

since C is 2 times more than G, the codon sequence

CCC CCG CGC GCC GGC GCG GGG that codes for peptide

PRO PRO ARG ALA GLY ALA GLY

% of GLY = 2 / 7 x 100 =28.57%

% of PRO = 2 / 7 x 100 =28.57%

% of ALA = 2 / 7 x 100 =28.57%

% of ARG = 1 / 7 x 100 =14.28%

now dinucleotide GC were used to make RNA and translated

now new codon will be

GCG CGC GCG CGC and so on which codes for amino acid

ALA ARG

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