4. (8 points) The ribonucleotides C and G were mixed together with C 2X more abu
ID: 87513 • Letter: 4
Question
4. (8 points) The ribonucleotides C and G were mixed together with C 2X more abundant than G. The resulting RNAs were translated in a cell free system.
A. (5 points) Give the amino acids and the relative percentages of each that would be found after the translation. Show all reasoning/calculations.
B. (3 points) Suppose that di-ribonucleotides (GC) were used to make RNA and then translated in vitro. What would the results be – explain all reasoning and explain how this information could be combined with the information in part A above to try to deduce the genetic code, assuming you didn’t already know it.
Please help with how to answer and approach this question. Thank you!
Explanation / Answer
4)
a) In particular cellular RNA contain a large variety of modification which are usually introduced by post transcriptional modification (1,2).
b) To date more than 100 chemically distinct RNA modification have been identified in bacteria, Archacea, and eukaryotes (3,4).
c)The abundant modification RNA is methylation of 2'hydroxyl group of ribose moiety.
d)2'o methylated nucleotides are present in all major classes of eukaryotes RNA,but are best studied in RNA.
e)In human ribosome more than 100 2'-o-methylation sits have been mapped (5,6).
f)site specified 2' methylation of eukaryotic RNA is mainly guided by numerous small nucleotides RNA's which direct the enzymatic machinery required for methylation toward complementary target region of ribosome.
g)Those modification sits are highly conserved among vertebrates and mainly occurs in clustered .
h) However the detailed function of 2o methylation in RNA is not yet will understood.
3) Transcription and translation are the means by which in their genes.Because mainly identifical RNA copies can bE made from same gene and each RNA molecule can direct the synthesis of many identifical protein molecules.cells can synthesized a large amount of protein rapidly when necessary . But each gene can also transcribed and translation with different efficiency allowing the cells to make vast quantities of some proteins and tiny Quantities of others.
2)Three is minimum no of nucleotides as per condon needed for encode 20 amino acids.
a)20 Amino acids are encoded by combination of 4 nucleotides.
b)If a codon were 2 nucleotides the sit of all combinations could encoded only .
4×4=16 Amino acids
c) With 3 nucleotides the sit of all combinations can encoded.
4×4×4=64 Amino acids.
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