You have sequenced a short fragment of DNA and it reads (from 5’ to 3’ on templa

Question

You have sequenced a short fragment of DNA and it reads (from 5’ to 3’ on template strand): TACGGCTATCGA           

           

            a)         Give the sequence if the non-template strand of DNA.

            b)         Give the mRNA transcript for this fragment.

            c)         Give the appropriate amino acid sequence that would be translated from this fragment (3 letter code is fine).

Second Base UUU7 Phe 11 UCC | Ser ||URA UAU7 Tyr 11 UGC UUU UCU UGU UUC- UAC UUA- UCA UAA Stop-UGA Stop | | | A Leu UUG UCG- UAG Stop | | UGG Trp11 G CCA | Pro || CACHis 11 CGUw CCU CAU CUC | Leu |ICCA CCC | Pro || CAA-1 Gin 11 CGG CGC C CUA CGA CUG CCG- CAG CGG- AUU ACU AAU AGU Asn Ser AUC | Ile ACC AAC AGC Thr AUA ACA AGA Lys AUG Met or Met or | | | ACG- AAG AGG Start GUU- GCU GAU GGU- Asp | GGC GUC GCC GAC GAGGlu 11 GGA | Gly Val Ala G11 GUA GCA GAA GUG- GCG- GGG aseg p41 CAG UCAG UCAG UCAG S-T UCAG GG- AAAA UC AA !|U C A -U AAAA ase8 1si_

Explanation / Answer

5' TACGGCTATCGA 3' DNA sequence

non-template strand sequence or coding DNA sequence

3'ATGCCGATAGCT 5' OR 5' TCGATAGCCGTA 3'

now mRNA sequence is similar to the non-template strand but with a slight difference. instead of T, U is present.

5' UCG AUA GCC GUA 3'.

Ser Ile Ala Val

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