Briefly explain how would create a tomato variety that is resistant to frost dam

Question

Briefly explain how would create a tomato variety that is resistant to frost damage because it has an antifreeze gene discovered in a plant in Antarctica. include (a)identify the specific type of vector you would use (b) explain how would isolate the salt tolerant gene from the source and get it into the corn genome (c) what the host cell for pedigree below, state what type of inheritance pattern it is most likely representing And you must explain your reason for the inheritance pattern. select auto dominant-auto recessive_x' link dominant_x link ressisive or mitochondrial.

Explanation / Answer

1. I would take the concerned variety of tomato and be using a suitable bacterial plasmid as a vector

so that I can insert the desired antifreeze protein gene from the plant of Antarctica and with the help of many molecular techniques available in genetic engineering I would incorporate in the genetic machinery that carries out transcription and translation a) pT7Blue vector would be my choice

b) isolation of salt-tolerant gene would be done with the help of Genetic markers and incorporation with the help of suitable vector

2. since the chart shows that both the genders are affected its autosomal trait and trait is hidden in some progeny so its clear that is recessive therefore the given chart is the autosomal recessive trait.

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