The aminoacid glycine is often used as the main ingredient of a buffer in bioche
ID: 786116 • Letter: T
Question
The aminoacid glycine is often used as the main ingredient of a buffer in biochemical experiments.
The amino group of glycine, which has a pKa of 9.6, can exist either in the protonated form ( -NH3+) or as the free base (-NH2), because of the reversible equilibrium
R-NH3+ <->R-NH2 + H+
How much of 5 M NaOH must be added to 1 L of 0.1 M glycine at pH 9.0 to bring its pH to
exactly 10.0?
The part I'm stuck on is when you solve it out and have 0.25119 = (0.1-x)/x
How do you solve for x in this situation?
Explanation / Answer
0.25119 = (0.1-x)/x
then 0.25119x = 0.1-x,
x = 0.1/1.25119 = 0.08
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