The amino-acid sequence shown jn the following table was obtained from the centr

Question

The amino-acid sequence shown jn the following table was obtained from the central region of a particular polypeptide chain in the wild-type and several mutant bacterial strains: Each mutant represents a single nucleotide change from the original wild-type gene. For each mutant, explain what SINGLE mutation at the DNA level accounts for the change(s) in amino acid sequence Also, state in which codon and which position the mutation took place and then lulls characterize the mutation as a base-pair substitution mutation (transversion or transition, and missense. nonsense, etc ) or a frameshift mutation. Figures 6.2 (p. 104). 6.7

Explanation / Answer

a. Wild- type sequence is already given. Comparing the rest we get the following results.

b.. 1. Pro to His- Change in the middle base, C is changed to A. Missense mutation.

2. Thr to His- Change in first two bases. Transversion mutation

3. Val to Gly- Change in middle base. Missense mutation

4. Thr to Asp- Change in first two bases. Transition mutation

5. Again, Thr to Asp- Change in first two bases. Transition mutation

6. Arg to Thr- Change in first two bases. Transversion mutation

7. Trp to Val- Change in bases. Frameshift mutation

c. Val to met- Change in first base. Missense mutation

d. Peptide not completely made. Nonsense mutation.

e.  Peptide not completely made, abruptly stopped. Nonsense mutation

f. Thr to Ser- Change in first base. Missense mutation

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