You are digesting a protein 625 amino acids long with the enzymes Factor Xa and

Question

You are digesting a protein 625 amino acids long with the enzymes Factor Xa and thrombin, which are proteases that bind to and cut proteins at particular short sequences of amino acids. You know the amino acid sequence of the protein and so can draw a map of where Factor Xa and thrombin should cut it. You find, however, that treatment with each of these proteases for an hour results in only partial digestion of the protein, as summarized under the figure. List the segments [A-E] of the protein that are most likely to be folded into compact, stable domains, and briefly explain (1 sentence] why you reached that conclusion.

Explanation / Answer

1. Factor Xa is a serine protease that is produced by activation of the zymogen. factor X by other the intrinsic or extrinsic factorr tenase complexes factor Xa is the enzyme component in the prothrombinase complex, which catalyzes the rapid conversion of prothrombin to thrombin.

2. Although factor Xa alone can convert prothrombin to thrombin,assembly of the prothrombinase complex results in a 300,000 fold increase in the rate of prothrombin conversion.Factor Xa activity is down-regulated by inactivation of the cofactor, factor Va or by direct in inhibition of factor Xa by its principal inhibitor.

Related Questions

Navigate

• Browse (All)
• Browse Y
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.