You are digesting a protein 625 amino acids long with the enzymes Factor X_a and

Question

You are digesting a protein 625 amino acids long with the enzymes Factor X_a and thrombin, which are proteases that bind to and cut proteins at particular short sequences of amino acids. You know the amino acid sequence of the protein and so can draw a map of where Factor X_a and thrombin should cut it. You find, however, that treatment with each of these proteases for an hour results in only partial digestion of the protein, as summarized under the figure. List the segments (A-E) of the protein that are most likely to be folded into compact, stable domains, and briefly explain (1 sentence) why you reached that conclusion.

Explanation / Answer

1. The segments digested with Factor Xa would be 50,75,100,175,250 length each

The size of segments digested with Thrombin are 50,80,245,250.

2. The chain of 625 amino acid proteins and proteases are digested with strong detergents and urea. The urea at a high concentration of 4M digests the 625 amino acid proteins that are exposed leaving the folded proteins with varied fragments according to length wise.

Related Questions

Navigate

• Browse (All)
• Browse Y
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.