I have an immunobiology question for you that is based on data analysis. I apoog

Question

I have an immunobiology question for you that is based on data analysis. I apoogize for the wordiness of this post. I am stuck right now! I seem to be having the biggest problem with the final question: Summarize what this data tells you about Odd, and briefly outline an additional experiment you would perform to further investigate this interesting gene. What type of experiments could I do!! I was informed to look for a way to measure whether the Odd gene secretes a protein or exists on a cell surface receptor.

Here is some background information:

In an effort to better understand the basis for affinity maturation observed during humoral responses, an investigator knocked out a gene called Odd in one strain of an IgH congenic pair. Thus, the Odd knockouts (-/-) bear the IgHx allotype and the wild-type (wt) bear the IgHz allotype. He immunized these mice with DNP10- KLH and monitored various aspects of the response (Table 1). He also made reciprocal chimeras by adoptively transferring marrow stem cells from Odd KO donors, wild-type donors, or 50:50 mixtures of KO and wt stem cells into lethally irradiated recipients. He immunized these chimeras after reconstitution, and assessed the affinities of IgG antibodies at d21 post immunization (Table 2).

Here are the questions I am supposed to answer:

1. What conclusions do you draw from the data in Table 1?

2. What do you conclude from the reciprocal chimeras (rows 3 and 4) in Table 2?

3. What additional conclusions does the mixed chimera afford?

4. Summarize what this data in toto tell you about Odd, and describe a possible experiment you would preform to further investigate this gene.

Table 1: Characteristics of DNP-specific immune responses in normal and Odd KO mice Characteristics Avg GCs per spleen nd 7:3 Day post Total Mouse mmunization Antibody KA (M) Average gM IgG mc nd 85 50 30 nd 20 165 430 100 ±18 wt 14 21 10 10 n.d 12±4 480+24 105±17 190±18 80 50 25 15 160 450 6±3 52+4 12±4 14 10 21 10 Table 2: Affinities of lgG DNP-specific serum antibodies at day 21 in reciprocal and mixed marrow chimeras

Explanation / Answer

According to the table 1, the odd gene has no change in average KA from day 0 to 21, while in wildtype (wt) the average KA has gradually increased.

According to table 2, the wt produced IgHZ, while odd produced IgHX. The odd containing wt genetic material produced IgHZ. Similarly, the wt containing odd genetic material produced IgHX. In case of 50:50, mix of both types produced both IgHZ and IgHX in equal proportion.

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