The gene encoding a hormone necessary for insect development contains DNA bindin

Question

The gene encoding a hormone necessary for insect development contains DNA binding sites for three gene regulatory proteins called A, B, and C. Because the binding sites for A and B overlap, A and B cannot bind simultaneously. You experimentally create a mutation in the binding sites for each of the proteins and measure hormone production in cells that contain equal amounts of the A, B, and C proteins. The results of your studies are summarized below (+ normal site, - mutation disrupts binding of protein) Binding Site        Hormone production A+B+C+               off A-B+C+               off A+B-C+               off A+B+C-               low level A-B-C+               off A+B-C-               low level A-B+C-               high level A-B-C-               off A) Protein C is a repressor/activator of transcription? B)Protein A is a stronger/weaker activator of transcription than Protein B? C)C is able to prevent activation by A only/B only/both A and B? D)Between the A protein and B protein, the A protein/B protein is more competitive for the overlapping binding site? The gene encoding a hormone necessary for insect development contains DNA binding sites for three gene regulatory proteins called A, B, and C. Because the binding sites for A and B overlap, A and B cannot bind simultaneously. You experimentally create a mutation in the binding sites for each of the proteins and measure hormone production in cells that contain equal amounts of the A, B, and C proteins. The results of your studies are summarized below (+ normal site, - mutation disrupts binding of protein) Binding Site        Hormone production A+B+C+               off A-B+C+               off A+B-C+               off A+B+C-               low level A-B-C+               off A+B-C-               low level A-B+C-               high level A-B-C-               off A) Protein C is a repressor/activator of transcription? B)Protein A is a stronger/weaker activator of transcription than Protein B? C)C is able to prevent activation by A only/B only/both A and B? D)Between the A protein and B protein, the A protein/B protein is more competitive for the overlapping binding site?

Explanation / Answer

a) Protein C is a repressor b) Protein A is a weaker c) C is able to prevent activation by both A and B d) Between the A protein and B protein, the A protein

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