#40- indentify the minimum amino acid numbers and the name of the domain require
ID: 59268 • Letter: #
Question
#40- indentify the minimum amino acid numbers and the name of the domain required for ubiquitination:
and what do C34A and H53A show?
The figure shows E1, E2, GST-ARDI (1-574), GST, and GST-cytohesin-1 (C-1) added to proteins that can be ubiquitinated and run on a gel. The asterix denotes heat-inactivated protein. The samples were incubated with an anti-ubiquitin antibody 3. This is a picture of (native, SDS-PAGE, westermy notile native- S-PAGE, western)? E, western)?_ngtive 35. What proteinsare required for ubiquitination in this experiment? -574 GST EL E2 136. Why is Lane 3 a smear? (Hint: similar to the C-1 36, why is Lane 3 a smear? (Hint: similar to the Endo H question #16) kDa 220- 7. Why were GST and C-1 added to Lanes 1 and 2? 90- 38. What was the purpose of the heat inactivation? 39. What type of ubiquitinating enzyme is ARDI (EI, E2, E3)? The researchers mixed some of the ARDI constructs from the first figure to test their ability to facilitate ubiquitination. Using the gel to the left, they determined which domains are essential for ubiquitination. 40. Identify the minimum amino acid numbers and the name of the domain hicbdo the min ano acid numbers and the me kDa required for ubiquitination: 220- 00- What do C34A and H53A show?Explanation / Answer
39. ARD 1 is member of ADP ribosylation family i.e. ARF.
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