#4 For the following four strains of the flu, identify how they are different fr

Question

#4 For the following four strains of the flu, identify how they are different from the typical strain. ALFirst: Translate the typical HA RNA. B) Then: for the new mutant strains 1-4, indicate a) where the mutation is and b) what kind it is. Possibilities are: missense (Change in amino acid Show the change.) nonsense (Change to a stop codon. Indicate the changed codon.) frame shift (Addition or subtraction of a nucleotide. Write the new amino acids.) silent (Nucleotide change that does not result in an amino acid change. Indicate the nucleotide that is changed.) deletion mutation (Removal of an entire amino acid(s)). Typical HA RNA 5' UAACCAUGAAGACUAUCAUUGCUUUGAGCUACAUUC 3' Strain #1 RNA 5' UAACCAUGAGGACUAUCAUUGCUUUGAGCUACAUUC 3' Strain #2 RNA 5' UAACCAUGAAGACUAUCAUUGCUUAGAGCUACAUUC 3 strain #3 5' UAACCAUGAAGACCAUCAUUGCUUUGAGCUACAUUC 3' strain #4 RIA 5' UAACCAUGAAGACAUCAUUGCUUUGAGCUACAUUC 3 #5 Based on the information you have, which of the four strains is most likely to be the one causing the illness? WHY??2 (HINT: Review the flu fact sheet.)

Explanation / Answer

Amino acid sequence of Typical HA RNA

UAACCAUGAAGACUAUCAUUGCUUUGAGCUACAUUC

Note - Upon translating the RNA I am getting Stop P Stop R L S L L Stop A T F which doesn't make sense. So I am removing the first U and translating the new mRNA. If you to use the original sequence it is at the end of the answer.

N H E D Y H C F E L H

Strain 1 RNA

RNA

UAACCAUGAGGACUAUCAUUGCUUUGAGCUACAUUC

The underlined codon is mutated.

N H E D Y H C F E L H

Upon mutation, there is no change in the translated amino acid

So mutation is a silent mutation.

Strain 2 RNA

U AAC CAU GAG GAC UAU CAU UGC UUA GAG CUA CAU UC

N H E D Y H C L E L H

Phenylalanine is changed into leucine. So mutation is missense mutation.

Strain 3-

U AAC CAU GAG GAC CAU CAU UGCUUUGAGCUACAUUC

N H E D H H C F E L H

Phenylalanine is changed into Histidine. So mutation is missense mutation.

strain 4-

UAACCAUGAAGACUUAUCAUUGCUUUGAGCUACAUUC

Here Insertion of U takes place.

SO mutation is frameshift mutation

New Peptide -

N H E D L S L L Stop A T F

MUtation leads to the introduction of the stop codon.So mutation is called non-sense mutation

5- The strain that is most likely to cause illness will be strain 1 as its sequence is same as that of the typical HA mRNA  

Strain 1-

Stop P Stop R L S L L Stop A T F

changed into

Stop P Stop G L S L L Stop A T F

Arginine is changed into glycine. So mutation is missense mutation.

Strain 2-

Stop P Stop G L S L L R A T F

There no mutation which can change stop codon into amino acid coding codon

Strain 3-

Stop P Stop G P S L L Stop A T F

Strain 4

Stop P Stop G P I I A L S Y I

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