Protein Sequencing. Determine the peptide sequence from the provide peptide sequ

Question

Protein Sequencing. Determine the peptide sequence from the provide peptide sequencing info below. ( Give explanation on how you got your sequence)

1. a decapeptice ( 10-residue peptide) called Fp has been found to have anticancer activity, containing the following amino acis residues, Arg, Asp, Phe, Cys, Gly, Ala, and Met.

2. Oncecycle of Edman degradation of the intact FP yields 2 mol of PTH-aspartate per mole of FP

3. Treatment of FP with 2-mercaptoethanol (used to reduce the disulfide bond and break the disulfide linkage peptides) followed by the addition of trypsin [cleave at Arf (C)] yields three peptides with the composition of: (Ala, Cys, Phe,), (Arg, Asp) and (Asp, Cys, Gly, Met, Phe). The intact tripeptide containing (Ala, Cys, Phe) yields PTF-cysteine in the first cycle of Edman degradation.

4. Treatment of 1 mol of FP with carboxypeptidase (which cleaves the C-terminal residue from peptides, i.e., cleave the last amino acid from carboxyl terminal) yields 2 mol of phenylalanine.

5. Treatment of the intact pentapeptide (Asp, Cys, Gly, Met, Phe) with BrCN [cleave at Met (C) yields two peptides with the composition of (Met, Asp) and (Cys, Gly, Phe).The (Cys, gly, Phe) peptide yields PFH-glycine in the first cycle of Edman degradation.

6. Summary of all of your conclusion and presentation of correct sequence.

Explanation / Answer

According to point 2, the first amino acid is aspartic acid since edman degradation is done to know the N terminal amino acid.

According to point 4 the last amino acid is phenylalanine.

According to point 5, the sequence of the first amino acids is Asp-Met. The N terminal amino acid of the tripeptide in point 5 is glycine.

The sequence is Asp-Met-Gly-Phe-Cys-Asp-Arg-Cys-Ala-Phe

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