Protein Sequencing Question. Determine the sequence of a peptide consisting of 1

Question

Protein Sequencing Question. Determine the sequence of a peptide consisting of 14 amino acids on the basis of the following data:
Amino acid composition: (4S, 2L, F, G, I, K, M, T, W, Y)
N-terminal analysis of full peptide: S
Carboxypeptidase digestion: L
Trypsin digestion: (peptide composed of 3S, 2L, F, I, M, T, W) and another composed of (G, K, S, Y)
Chymotrypsin digestion: (one peptide composed of (F, I, S), another of (G, K, L), another of (L, S), another of (M, T), another of (S, W) and another of (S, Y)
N terminal analysis of the (F, I, S) peptide is S
Cyanogen bromide treatment of the full peptide results in one peptide composed of (2S, F, G, I, K, L, M*, T, Y) and another composed of (2S, L, W)
M*, methionine detection as homoserine.

Explanation / Answer

First amino acid: S Last amino acid: L Cyanogen bromide cleavage: M is 10th position, C-terminal residues are: (2S,L,W) Amino-terminal residues: (G,K,S,Y), tryptic peptide, ends in K Amino-terminal sequence: SYGK Chymotryptic peptide order: (S,Y), (G,K,L), (F,I,S), (M,T), (S,W), (S,L) Sequence: SYGKLSIFTMSWSL

Related Questions

Navigate

• Browse (All)
• Browse P
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.