Q1) The sample loading buffer mixed with the protein extract serves to: a) Help

ID: 56453 • Letter: Q

Question

Q1) The sample loading buffer mixed with the protein extract serves to:

a) Help the sample sink into the wells, because of the dense glycerold included

b) Adjust and maintain the pH of the sample, due to the pH buffer included

c) Provide a tracking dye that allows us to follow the progress of electrophoresis

d) Provide bromophenol blue which allows us to see the sample better during loading the samples

e) All of the above

Q2) In analyzing tyrosinase activity measuring absorbance at 475nm directly measures:

a) Blue light taken up by dopachrome

b) Blue light taken up by DOPA

c) Orange light takn up by DOPA

d) Orange light taken up by dopachrome

e) Blue light reflected by dopachrome

f) Blue light reflected by DOPA

Q3) The loss of function of an enzyme due to unfavorable enviornmental conditions is termed __________?

a) Dissociation

b) Disarray

c) Deactivation

d) Denaturation

e) Deformation

Q4) In addition to gel electrophoresis, which of the following techniques are used in labs to seperate molecules based upon molecular size?

a) Spectrophotometry

b) Gel Filtration

c) Colorimetric Assay

d) Paper chromatography

e) All of the above

1. c) Provide a tracking dye that allows us to follow the progress of electrophoresis

2. (a) Blue light taken up by dopachrome which is orange in color

3. d) Denaturation because the nature of enzyme is destroyed

4. (e) All of the above can be used .

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