1. The following five proteins, which are listed with their molecular weights an
ID: 55343 • Letter: 1
Question
1. The following five proteins, which are listed with their molecular weights and isoelectric points, were separated by SDS-PAGE. Give the order of their migration from the top to the bottom of the gel.
2. If the five proteins listed above were separated in an isoelectric- focusing experiment, what would be the order of their distribution between the positive (+) and negative (-) ends of the gel?
Protein Name Molecular weight (daltons pI Alpha-antitrypsin 45,000 5.4 Cytochrome c 13,400 10.6 Myoglobin 17,000 7.0 Serum albumin 69,000 4.8 Transferrin 90,000 5.9Explanation / Answer
1. The order of migration depends the molecular weight of the protein. The migration of the proteins from the top to the bottom of the gel are as follows:
2) The isoelectric focusing depends on the isoelectric point and amount of charge
Sl. No. Protein Name Molecular weight (Daltons) 1 Transferrin 90000 2 Serum albumin 69000 3 Alpha-anititrypsin 45000 4 Cytochrome c 13400 5 Myoglobin 1700Related Questions
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