1. The following five proteins, which are listed with their molecular weights an

Question

1. The following five proteins, which are listed with their molecular weights and isoelectric points, were separated by sDs-polyacrylamide gel electrophoresis. Give the order of their migration from the top (the point of sample application) to the bottom of the gel. Molecular weight p a. a-Antitrypsin c. Myoglobin d. Serum albumin e. Transferrin 45,000 13,400 17,000 69,000 90,000 5.4 10.6 7.0 4.8 5.9 Top Bottom If the proteins were separated in an isoelectric-focusing experiment what would be their distribution between the positive +) and neg () ends of the gel? Indicate the high and low pH ends. cathode (-) (+) anode

Explanation / Answer

1. The order of migration depends the molecular weight of the protein. The migration of the proteins from the top to the bottom of the gel are as follows:

2) The isoelectric focusing depends on the isoelectric point and amount of charge

Sl. No. Protein Name Molecular weight (Daltons) 1 Transferrin 90000 2 Serum albumin 69000 3 Alpha-anititrypsin 45000 4 Cytochrome c 13400 5 Myoglobin 1700

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