You use the Bradford (coomassie) assay to determine the concentration of a histo

Question

You use the Bradford (coomassie) assay to determine the concentration of a histone protein. You use bovine albumin as your standard. You determine the histone protein concentration to be 5 mg/ml.

When you use the same histone protein as your standard, you determine the histone protein concentration to be 3 mg/ml.

a. The histone protein has fewer basic amino acids than albumin.

b. The histone protein has more acidic amino acids than albumin

c. The histone protein has more basic amino acids than albumin

d. The histone protein contains more methionine than albumin

Explanation / Answer

Note that

--- >The histone protein has fewer basic amino acids than albumin.; therefore choose A

Note that the Braford assay is subjective, i.e., dependent on the amino acid composition of the measured protein.

Therefore, when you changed the standard to the histone, you will have a lower concentration as expected

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