2. What is/are the differences between the chloroplast sample(C) and your total

Question

2. What is/are the differences between the chloroplast sample(C) and your total protein sample ("T")?

3. What is/are the differences between your chloroplast sample and your soluble sample ("S")? As you did for question 3, provide an explanation for any differences or lack thereof.

T=5.0 mL of Isolation Medium,5 grams of grinded spinach leaves,

C= whats left on bottom after centrifugation(This process pellets any additional chloroplasts and other organelles, leaving only soluble (i.e. cytoplasmic) protein in the supernatant. ) of sample T.

S=supernatant from C

Can you help me with these questions? Q..Q

Explanation / Answer

The cell is mainly composed of biomolecules- Carbs/Lipids/Proteins.

All these have different sedimentation coefficient, if you will CENTRIFUGE them. T could be the Total proteins separated after centrifugation. But this protein consists of - cytoplasmic proteins/membrane bound proteins/integral proteins/proteins present associated with organelles, etc.

Now C is the chloroplast, which is collected at the bottom and S are the other soluble proteins found as supernatant after centrifugation.

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