A freeze-fract molecules under the influence of an electric field in known as C
ID: 3167302 • Letter: A
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A freeze-fract molecules under the influence of an electric field in known as C chromakography D clectrophoresis E. centrifugaion 11. In all the electrophoresis A. (-) electrode B. (+) electrode experiments you performed, the scrum samples were loaded on the E. red electrude C. D. anode electrode both (+) & (-) electrodes sample. A. True s buffer is critical because it determines the net charge on the protein molecules in the B. False 13. In strong acid solutions, amino acid A. True s are positively charged and migrate in an electrie field to the negative electrode B. False Virtual ELISA 14. Which of the following instruments tubes or to other centrifuge tubes? were used to transfer a specific amount of each serum samples to different E centrifuge B. pipertor C. analytical balance D. watertbath of the following is a potential problem that could have occurrod in the virtual ELISA? A. reagent selections D. ELISA plate not washed properly D. temperature & time settings were not set properlyall of the above C. volume measarements were incorrect 16. Wh at was the purpose of setting up a positive control in the ELISA plate? A. to increase the number of well solution B. to use all of the reagents C.to verily that the procedure is optimized and working D. Il with solution to monitor the temperature and time Electrophoretic Separation of Proteins 17. Which of the following components were found in all the protein samples to ensure that the samples layered smoothly at the bottom of the sample wells? A. glycerol B. acetie acid C. mcthylene blue D. Coomassie blue E. bromophenol blue 18. s. Which of the following types of stabelizing supports did you use in the electrophoretic experiments such as the separation of serum proteins? A starch B. nitrocellulose C. agars D, sugar 9. During he electrophoresis, the larger proteins move slower and are found at the top of the gel while the smaller proteins move faster and are found at the hottoem of the gel A. True B. False What was the tracking dye found in the sample baffer as the samples were loaded into the well of gel? A. water .glycerol C. antibodies D, TBS E. bromomphenol blue 21. Which protein possessed a positive charge and migrated at the negative electrode at pll 8.67 A. myoglobin B. hemoglobin C. cytochrome C D serum albumin E. vitamin B12 Which protein possessed a negative charge and migrated the slowest to the positive electrode at ph 3.6? D, serum albumin E vitamin B12 Which protein possessed a negative charge and migrated the fastest to the positive electrode at pH 8.6 A. myoglobin B. hemoglobin C. cytochrome C D. serum albumin E. vitamin B12 denification and Evolutionary Relationships of Serum Proteins by Western Press-Protein Blot 24 The enzyme that converted chloronapthol (colorless) to chloronapthol (colored product) was A. catalase B. peroxidase C. Coomassie blue D. gelatin E. lactate dehydrogenaseExplanation / Answer
10) Movement of charged molecules under the influence of an electric field is Electrophoresis. These molecules move towards the positive electrode. The movement of the molecules through the gel will separate the particles based on molecular weight.
Right option is D. electrophoresis.
11. Sample in electrophoresis is loaded on the negative electrode. The serum proteins have a net negative charge. They migrate to the positive electrode under the influence of the electric field.
Right option is A. (-) electrode.
12. The pH of the electrophoresis buffer determines the net charge on the protein. If the pH is above the isoelectric point of the protein, it will have a net positive charge. If the pH is at the isoelectric point, then the protein will have no charge. However, if the pH is less that the pI, than the protein will have a net negative charge.
Option is A. True.
13. The migration of the amino acid in electrophoresis depends on the pH of the medium. If the amino acid is positively charged that it will migrate towards the –vely charged electrode. Amino acids are positively charged at pH below their isoelectric point.
Amino acid has a positively charged amino group and a negatively charged carboxyl group. In and acidic solution, H+ ions are added to NH2 group and COO- group. Hence, the amino acid will be positively charged, as NH2 becomes NH3+ while COO- becomes COOH. Hence, the amino acid moves towards the negatively charged cathode.
Option is A. True.
14. A pipette is the instrument that is used to transfer specific amount of substance from one tube to another. It has calibration that can pick up specific quantity of substance or volume from one tube and transfer into another tube.
Right answer is B. pipette.
15. Enzyme-linked immunosorbent assays (ELISA) detect and quantify biological molecules. However, error may occur due to following reasons:
a) The wrong antibody was selected. Hence, it cannot bind to the antigen giving error.
b) If the Elisa plate is not washed properly, then nonspecific binding may occur.
c) If the temperature is high, the enzyme may get denatured. Enzyme activity depends on the temperature. If the reagents are incubated for longer time, no specific binding may occur.
d) If the amount of antibodies added is less, then the amount of reaction formed will vary.
Right option is E. All of the above.
16. The positive control is added to ensure that the procedure works optimally. If there is no signal in positive control, then the experimental sample results cannot be accurate.
Right option is C.
17. Glycerol acts as a proteins stabilizer. It has a high density. Hence, it will make the protein denser, and will allow the protein sample to settle at bottom of the well.
Right option is A. Glycerol.
As per Chegg’s rules, only one question needs to be answered.
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