1. Two pure breeding plants with the genotypes ddPP and DDpp are crossed togethe
ID: 282776 • Letter: 1
Question
1. Two pure breeding plants with the genotypes ddPP and DDpp are crossed together to generate Fl progeny with the genotype DdPp. F1 individuals were testcrossed to a homozygous parental strain (ddpp). The following data were obtained: Genotype observed # of progeny Expected # of progeny 0 DdPp ? Ddpp Ddpp ddPp 190 210 310 290 Total: 1000 ? I00o A. In the table above, fill in the values for the expected number of progeny for each genotype based on the hypothesis that D and P are unlinked. B. What is the calculated ?2 value? C. How many degrees of freedom are there? 3 D. Are D and P linked or unlinked? (Use the x2 table attached below). E. Briefly explain how the x2 analysis supports your conclusion. ? 0.999 0.995 0.999 975 . 09 0.5 0.1 0025 0.01 005 0.001 10.000 0.000 0.000 00 0.004 16 0.455 2.06 3.841 5.024 6.635 7.879 10.828 2 0002 0.010 0.002 0.051 0.103 0.211 1.386 4605 599. 7.378 %210 10.597 13.815 9.348 11.345 12.838 16.266 4 0.091 07 091 484 0.711 1.064 3.357 7.779 9.488 11.143 13.277 14.860 18.467 S 0.210 CAL2 0.210 0831 1.145 1.6?4.351 3236 11.070 12.833 15.086 15.730 20.515 0.024 007220.216 0.352 584 2366 6251 115 73.761 79.69273.761 7.213 91.242 96043 11334 134.813 14100 146.571 153.191 157.808 167.610 116 74558 80.522 7455s ss.os4 92.134 96.958 ?15.334135.898 142.138 i47.700 is 344 158.977 158.813 117 75.356 81.353 75356 88955 93.026 97.874 116334 ?6.982 141246 148829 1554% 160,146 170.016 118 76.155 82.las 7.155 8.827 93.918 98.790117.134 13a066 ??15410357 1566ts s1.3M.171.217 119 76.955 83.018 76.955 90.700 94.811 99.707 118.334 133.149 14.461 1S1.084 157.800 162481 172.418 120 77.755 83.85277.755 91.573 95.0 100.624 11334 140.233 146.7 152.211 158950 163.648 173.617Explanation / Answer
Answer :
Chi square calculation looks correct.
X2= (190-250)^2/250+(210-250)^2/250+(310-250)^250+(290-250)^2/250
=14.4+6.4+14.4+6.4= 41.6
P value is 0.0001 when Chi square is 41.6 and df =3
This value is very much statistically significant. The less the p value there is the no linkage or association between the genes. So D and P genes are not linked
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