,Q6 (4pts) Describe the use of the culture collection in interpreting an uncultu
ID: 280849 • Letter: #
Question
,Q6 (4pts) Describe the use of the culture collection in interpreting an uncultured 16S rRNA sequence /Q7 (5 pts) If you were examining the changes in cyanobacterial activity that occurs between day and night would you use A) cloning sequencing and phylogenetic analysis of 16S rRNA genes: B) metagenomics C) metatranscriptomics; clearly explain why and what you would expect to find in the data Q8 (5pts) Pretend that you are an environmental microbiologist employed by a consulting firm hired to create a bioremediation plan for an aromatic carbon pollutant in soil at a former industrial site. As a first step your manager has asked you to isolate bacteria from the site's soil that would be capable of degrading the pollutant Describe the microbial growth media you would design and the steps you would take to enrich and isolate a pure culture capable of degrading the pollutant. Describe why you would take each stepExplanation / Answer
Q7. I will choose metatranscriptomics analysis.
Cloning and 16S rRNA genes are used for bacterial identification, classification, and phylogenetics related research.
Metagenomics is not useful because total genome remains unchanged during favorable as well as unfavorable condition. So, we cannot predict genes related to that phenomena using genomics.
Metatranscriptomics will work fine because transcriptome data is the mRNA data which are transcribed from DNA and the proteins are expressed in that specific condition. If we compare the transcriptome of day and night of the cyanobacterial colony we can acquire details about genes involved in that incident and their mechanism and probable pathway of action.
Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.