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Effectiveness of Disinfectants Data Collection Part 1 Observe, measure, and comp

ID: 280080 • Letter: E

Question

Effectiveness of Disinfectants Data Collection Part 1 Observe, measure, and compare the zone of no growth (inhibition), if any, around the disk for each disinfectant or antiseptic for both organisms. Record the diameters (in millimeters) of the zones of inhibition a table. Fill in the following table. Write the DIAMETER of the Zone of Inhibition (ZOI) in millimeters Disinfectant/ Antiseptic S. aureus ZOI E. coli ZOI 10% Toothpaste 10% leaking loo%| Mouthwash 29 Hydrogen Vin eaar 12 peroXide NOTE: Do not be disappointed if your favorite antiseptics or disinfectants d not appear to-be as bactericidal as advertised. The amount of disinfectant (or antiseptic) that the filter paper disk will absorb and the amount of diffusion into the medium surrounding the disk are dependent on the moleculd weight of the disinfectant. The rate of diffusion is directly proportional to molecular size. So do not discard, for, example, your favorite mouthwash on the basis of this activity.

Explanation / Answer

1. False
2. False - Antiseptic used in human tissue where as disinfectant used in materials for cleaning.
3. True
4. True
5. True
6. True
7. False
8. False - Plastic are not able to autoclave.
9. False

11. Exposure time, Concentration of disinfectant, pH, temperature, Concentration of microorganisms.

12. The microorganisms cell wall has different composition.
Gram positive - Thick peptidoglycan layer
Gram negative - Thin peptidoglycan layer, middle plasma membrane and outer membrane
Fungus - Chitin

13. Gram-positive bacteria are more susceptible to chemical disinfectants because peptidoglycan layer
are easily degrade or solublize by disinfectant.

14. Mycobacteria or bacterial endospores are more resistant. There cell wall are not easily digestable.

15. Bactericidal - kills bacteria. and Bacteriostatic - inhibits growth of bacteria.

17. Bacterial endospores are not able to kill by disinfectant which cause illness to the patients.

18. Disinfectants are applied to non-living objects to destroy microorganisms that are living there. so proper disinfection of equipment used on patients is crucial because many patients who have been exposed to needlesly to contaminated equipment can contract diseases such as HIV.

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