1. Describe the experimental method used to generate the data shown in the figur

Question

1. Describe the experimental method used to generate the data shown in the figure. Describe how a western blot was performed.

http://www.jneurosci.org/content/21/6/1830.long#sec-1

-What cells/animals were used?

-How was the gel run?

-What specific primary and secondary ab were used?

-How was the gel visualized?

2. Describe the results (describe what you see in the figure).

Fig. 5 Striatum (putamen) huntingtin fragments detected with antibodies recognizing the N terminus, N terminal domain, and C-terminal domain of htt. Striatum (putamen) tissue homogenates from control (lanes 1, 3, 5) and HD (lanes 2, 4, 6) brains were analyzed by Western blots with anti-(1-17) serum (lanes 1, 2), N- terminal domain -250 210 -200 kDa 5 120-116 100 -66 -55 -36 -31 50 50 4 38 35 20 -21 Tissue: C HD Antibody: (1-17) C HD N-domain C HD C-domairn View larger version In a new window Download as PowerPoint Slide s antibody (lanes 3, 4), and C-terminal domain antibody (lanes 5, 6). Each laneof the gel (12% SDS-PAGE) contained identical amounts of homogenate protein (18 ug).

Explanation / Answer

mkllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllll.............................................................llllllllllllllllllllllllllllllllllllllllllllllllll...........................

Related Questions

Navigate

• Browse (All)
• Browse 1
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.