4 You have prepared lipid vesicles (spherical lipid bilayers) that contain Na\"-

Question

4 You have prepared lipid vesicles (spherical lipid bilayers) that contain Na"-t pomortion of the sole membrane protein. All of the Na-K pumps are oriented in such molecule that normally faces the cytosol is on the inside of the vesic molecule that normally faces the extracellular space is on the outside of the each pump transports one Na" ion in one direction and one K' ion in the each pumping cycle (see Figure below for how the Na-K' pump normally functions in plasma membrane). a way that the portion of the le and the portion of the vesicle. Assume other direction Predict what would happen in each of the following conditions: The solutions inside and outside the vesicles contain both Na" and K' ions but no ATP. B. The solution outside the vesicles contains both Na' and K' ions; the solution inside contains both Na" and K ions and ATP The solution outside contains Na"; the solution inside contains Na' and ATP url Na become clogged and The cycl wn p

Explanation / Answer

A. Since there is no ATP the pump will bind with Na+ from the inside but it will not be phosphorylated so it will not open and thus will not be able to release ATP outside. In short, it will not be able to function.

B. It will function properly as ATP is needed from inside not outside. So, ATP is available it can transfer the Na+ outside and K+ inside. It will work as follows.

It will take Na+ gets phosphorylated then transfers it outside then take K+ get dephosphorylated and then transfer K+ inside. And again take Na+ and then cycle resumes once again.

C. It will be able to transfer Na+ outside but it will not be able to transfer K+ inside. and it will get halted at step 3.

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