1) what are the ingredients in MSA, Hektoen and MacConkey supplying carbon and/o
ID: 273580 • Letter: 1
Question
1) what are the ingredients in MSA, Hektoen and MacConkey supplying carbon and/or nitrogen to the bacteria growing on the media?
2) Knowing which ingredients in MSA, MacConkey an dHektoen agar make the media selective or differential and knowing which bacteria grow or don't grow on these plates and if they grow, have some knowledge as to how they would look?
3)What does enzyme casease have in commonwith amylase?
4) How do you know these are exoenzymes rather than working in the cytoplasm?
5) Besides the term depolymerization (disassembly of DNA), What other term can be used to describe this process?
6) Difference term, oxidative organism vs. fermentative organism?
7) What is a monobactam & why/when is it used?
8) What is clavulanic acid & how/why is clavulanic acid used?
9) how can one relatively easily test for the ability of bacteris cell to produce a beta lactamase?
10) Typically, what kind of genetic element carries the gene for beta lactamases-is it on the chromosome, on a plasmid? Can be associated with transposons?
Explanation / Answer
Answer 1]
The ingredients in MSA, Hektoen and MacConkey supplying carbon and/or nitrogen to the bacteria growing on the media are as:
Ingredients of MSA=
enzymatic digest of Casein-is source of nitrogen,vitamin and carbon.
enzymatic digest of animal tissue--is source of nitrogen,vitamin and carbon.
beef extract- is source of nitrogen,vitamin and carbon.
sodium chloride
Ingredients in Hektoen supplying carbon and/or nitrogen to the bacteria growing on the media is= peptone.
Ingredients in MacConkey supplying carbon and/or nitrogen to the bacteria growing on the media is =
1.enzymatic digest of Gelatin.Casein and animal tissue : provides nitrogen ,vitamins ,minerals and amino acids essential for growth
2.Lactose: fermentable carbohydrate providing carbon.
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