PLEASE EXPLAIN HOW THEY GOT THE ANSWERS IN RED FOR PART A & B. THESE ANSWERS ARE

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PLEASE EXPLAIN HOW THEY GOT THE ANSWERS IN RED FOR PART A & B. THESE ANSWERS ARE FROM THE OFFICIAL ANSWER KEY, DO NOT REPLY SAYING OTHERWISE. SHOW WORK!! THANK YOU.

PLEASE EXPLAIN HOW THEY GOT THE ANSWERS IN RED FOR PART A & B. THESE ANSWERS ARE FROM THE OFFICIAL ANSWER KEY, DO NOT REPLY SAYING OTHERWISE. SHOW WORK!! THANK YOU.

11. (10 points) Your F+ strain has a single auxotrophic mutation (his-), is able to use both lactose and galactose, and is sensitive to erythromycin and resistant to streptomycin. Your F- strain has a single auxotrophic mutation (arg-), is unable to use either lactose or galactose, and is resistant to erythromycin and sensitive to streptomycin. Hfr strain A is derived from the F+ strain described above. The order of markers transferred by HfrA is: (first) his str arg gal ery lad ast a) Your master plate should select for the greater possible number of exconjugants. Assume you are using minimal media (MM). Circle ONE answer for each sentence The carbon source(s) on the master plate should be GlucoseI lactose galactose lactose & galactose The master plate should include erythromycinstreptomycin bothneither The master plate should include arginine histidine both neither You make replica plates of the following compositions. Assume each plate has glucose unless gal and/or lac is added instead. Plate 1 MM+ arg+ ery+gal Plate 2 MM tery + lac str Plate 3 MM t ery + his str Plate 4 MM arg+ gal + his Plate 5 MM tery +gal + his Plate 6 MM arg+ery + his Plate 7 MM +gal + his+ lac Plate 8 MM +gal lac+ str b) Which plate will have the most colonies? Plate 6_Which will have the least?_ Plate 2

Explanation / Answer

Answer a.

Answer b.

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