PLEASE EXPLAIN HOW THE RED ANSWERS IN PART B WERE DETERMINED. SHOW ACTUAL WORK.

Question

PLEASE EXPLAIN HOW THE RED ANSWERS IN PART B WERE DETERMINED. SHOW ACTUAL WORK.

PLEASE EXPLAIN HOW THE RED ANSWERS IN PART B WERE DETERMINED. SHOW ACTUAL WORK.

8. (12 points) Using P22 as a generalized transducing phage grown on an arg+pro- lact bacterial donor, a recipient strain of genotype arg-pro+ lac- is infected and incubated. Afterwards, 1000 arg+ transductants were selected. (Note: The alleles arg+ and pro+ are necessary to synthesize arginine and proline respectively. All bacteria have the ability to utilize glucose as a sugar source. The lact is necessary for utilization of lactose.) a. What media was used for the arg+ selection? (3 points) Minimal Medium+proline b. Replica plating is used to screen for the additional markers. The replica plates contain the following media (the presence of a supplement is indicated with a + and its absence with a -) The number of colonies on each replica plate were shown at the bottom. (4 points) Media Replica 1 Replica 2 Replica 3 Replica4 Arginine Proline Lactose Glucose 700 82 1000 # colonies Fill out the table below with the genotypes of all possible transductants and the number of each type recovered. Genotvpe arg+ pro+tlac+ arg+ protlac- arg+ pro-lac+ arg+ pro-lac- # colonies 698 80 220

Explanation / Answer

They all play an important role in genotype s and colonies. They are interdependent to each other

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