A generalized transducing phage is used to transfer genes from a his(+)met(+)phe

Question

A generalized transducing phage is used to transfer genes from a his(+)met(+)phe(+) donor strain of E. coli to a his(-)met(-)phe(-) recipient strain. When transductants selected on a medium containing histidine and methionine are tested for growth on other media, many more colonies are found on media supplemented only with histidine than on media supplemented only with methionine. No transductants survive on a basal medium. What is the correct gene order and which 2 genes are most closely linked?

Explanation / Answer

Answer. According to data provided only histidine and methionine. So if colony grows more on histidine compared to methionine then its possible that his gene is first then methionine. And no colony in basal media then it's means phe gene is far apart. So histidine and methionine gene are closely linked. The gene order is his(+) met (+) phe (+)

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