A generalized transduction experiment is set up so that a phage is carrying a do

Question

A generalized transduction experiment is set up so that a phage is carrying a donor strain with the genotype met bio thr. This donor strain is used to transduce a recipient E. coli strain with the genotype met bio thr. After transduction, the recipient population is plated on a minimal medium, containing only organic salts and a simple carbon source, supplemented with methionine and biotin. A large number of colonies are obtained. The wild-type genes indicate the bacteria do not need a supplemented minimal medium in order to grow. Any bacteria with mutant genes will require a supplement of methionine, biotin, or threonine to grow. The use of minimal medium without all the required nutrients is a type of marker selection. Marker selection utilizes the inability of some bacteria with mutant genotypes to grow on minimal medium by creating a medium that only select bacteria can grow on. Select the possible genotypes of the colonies that grew on this medium. met bio thr met' bio thr met bio thr met' bio thr

Explanation / Answer

The media is Suplemented with methionine and biotin. So the only nutrients bacteria have to make is threonine. As the recipient bacterium has faulty threonine gene so if colonies found in minimal media there must be threonine positive colonies occur via transduction.

So here are two possible genotype

1.met+ bio- thr+

2. Met- bio+thr+

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