A gene acquires a point mutation that eliminates its stop codon. Proteins are ex

Question

A gene acquires a point mutation that eliminates its stop codon. Proteins are extracted from a wild type strain and one with this mutation, and a western blot is performed with an antibody specific for this protein. What result are we going to see? Select the best answer.

A . The wild type and mutant bands will be identical

B. The mutant band will be found higher on the gel than the wild type band

C. The mutant band will be found lower on the gel than the wild type band

D. The mutant band will be found at the same spot of the gel but will be more intense

Explanation / Answer

Proteins are extracted from a wild type strain and one with this mutation, and a western blot is performed with an antibody specific for this protein. What result are we going to see?

C) The mutant band will be found lower on the gel than the wild type band

The protein in mutant strain will have larger molecular weight than wild type starin. So, we will see a lower band on gel.

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