1. We analyzed the charge on the RGD tripeptide as a function of pH, and found t
ID: 259840 • Letter: 1
Question
1. We analyzed the charge on the RGD tripeptide as a function of pH, and found that the peptide was neutral at the isoelectric point of 6.3.
A) What is the isoelectric point of an RGD protein that consists of 100 RGD sequences in a row?
B) Now consider a large protein that only contains R, G, and D amino acids, but not in equal numbers. The isoelectric point of the protein is measured to be 7.4. Can you determine the relative numbers of the three different amino acids in the protein? If so, what are they? If not, what information about the amino acid content can you obtain from the isoelectric point?
Explanation / Answer
The isoelectric point still remains the same and it is 6.3 itself.
The relative number would be 6.3, 7.4 and 8.5 in the protein. Isoelectric point shows the point at which a molecule does not carry any electric charge.
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