What is the fragment size that would result from a Eco RV/ Pst I digest of pGEX-

Question

What is the fragment size that would result from a EcoRV/PstI digest of pGEX-6P-2-insert?

Please explain :D

0..O GST requlato A map of pGEX-6P-2 ter, for inducible G 58. .989 GST CDS 205...211 tac 780 Forward 945 BamH 955 EcoRI 960 Smal 960 Xma 945. 982 multiple cloning site (MCS); o 965 Sall 970 Xhol 975 Notl EcoRV 4132 overproducing allele CDS 3334 evers mer pGEX6P-2.xdna-4985 nt 1346. 1351 beta-lactamase promoter regula 1393...2253 ampicillin resistance gene C 1934 Pst region necessary for plasmid r 2318...3014

Explanation / Answer

The entire plasmid size is 4985 nt.

Digesting the plasmid with pst1 and EcoRV will yield 2 DNA fragments.

EcoRV cuts at 4132 nt and pst1 at 1934, so

4132 - (no. of sequence before pst1 site) 1933 nt = 2199

4985 nt - 4132 EcoRV site= 853

2199 + 853 = 3052

Size of one DNA fragment is 3052 nt.

4985 nt - 3052 nt = 1933 nt.

The size of the other DNA fragment is 1933 nt.

Related Questions

Navigate

• Browse (All)
• Browse W
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.