DNA polymerase I of E. coli has the three activities listed below. Enter the let
ID: 252634 • Letter: D
Question
DNA polymerase I of E. coli has the three activities listed below. Enter the letter that describes each activity. (There is only one letter per activity)
3’à 5’ exonuclease___ 5’à 3’ polymerase___ 5’à 3’ exonuclease___
a.Recognizes the origin of replication
b. Removes the primers from the new DNA strand
c. Seals nicks in the sugar phosphate backbone of the new DNA strand
d. Adds dNTPs to the growing DNA strand
e. Removes mismatched bases from the new DNA strand
f. Recognizes specific termination sequences in the template strand
Explanation / Answer
3' to 5' exonuclease activity: e. remove mismatched bases from the new DNA strand. exonuclease activity is for proof reading of DNA strand. incorrect dNTPs are recognised and removed with correct base apir.
5' to 3' polymerase activity: d. adds dNTPs to the growing DNA strand. polymerisation is a main function of tag DNA polymerase. it adds new dNTPs in growing DNA strand.
5' to 3' exonuclease activity: c. seal nicks in the suger phosphate backbone of the new DNA strand. labelled DNA strand or dNTPs are used to seal the nick in suger phophate backbone by exonuclease activity which is used in fluoro or radio labbed tagging.
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