The gene for resistance to peanut leafspot disease was inserted into a pUC19 vec
ID: 22112 • Letter: T
Question
The gene for resistance to peanut leafspot disease was inserted into a pUC19 vector using the enzymes SpeI and HindIII. The map of the recombinant plasmid is shown above.
The inserted disease resistance gene is ____ bp long.
Restriction digests were performed and fragments were separated based on their migration in an electrical current. Assuming that all digests were complete, select all fragments that would appear on the gel if the recombinant plasmid was cut with the enzyme EcoRI. All sizes are given in base pairs.
625
250
2225
5500
1120
6500
2650
430
1000
1250
3275
2400
Fragments were then transferred to a nylon membrane. The inserted gene was used as a probe. Detection methods were used to visualize the location of the probe on the nylon membrane. Select all fragments that would be visualized on the nylon membrane if the enzymes SpeI and EcoRI were used to cut the DNA.All sizes are given in base pairs.
2225
430
1000
1250
5500
680
2650
6500
2400
625
3275
250
1970
Explanation / Answer
first one is 3790-680 = 3110, idk others
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